breast epithelial cell lines mcf10a  (ATCC)

Journal: Translational Cancer Research

Article Title: Expression analyses of fatty acid binding proteins (FABPs) in breast cancer subtypes: implications for immune modulation and clinical outcomes

doi: 10.21037/tcr-2025-aw-2483

Figure Lengend Snippet: Validation of FABP gene expression in breast cell lines. (A) Relative expression of FABP genes in MCF10A, MCF7, and MDA-MB-231 cell lines. Quantitative real-time polymerase chain reaction analysis of FABP4, FABP5, FABP6, and FABP7 expression levels in the non-malignant breast epithelial cell line MCF10A and breast cancer cell lines MCF7 and MDA-MB-231. Data are represented as log 10 fold changes relative to MCF10A, with GAPDH as an internal control. (B) Comparison of FABP gene expression between MCF7 and MDA-MB-231 cell lines. Relative expression levels of FABP4, FABP5, FABP6, and FABP7 in MDA-MB-231 compared to MCF7. Data are expressed as log 10 fold changes calculated using the ΔΔCq method. ***, P<0.001 as determined by Student’s t -test. FABP, fatty acid-binding protein.

Article Snippet: The breast cancer cell lines MCF7 and MDA-MB-231, along with the non-malignant breast epithelial cell line MCF10A, were obtained from the American Type Culture Collection (ATCC).

Techniques: Biomarker Discovery, Gene Expression, Expressing, Real-time Polymerase Chain Reaction, Control, Comparison, Binding Assay